Hi I am Ralf,

I am working on water dependent hydroxylases of molybdenum-cofactor containing dimethyl sulfoxide reductases (DMSOR) enzyme family from anaerobic bacteria. They are involved in the degradation processes of many toxic compounds, such as steroids, aromatics or even alkanes under anaerobic conditions. I am developing enzyme assays to follow the mechanism of such water insoluble compounds in an aqueous environment. For substrate identification I apply various chromatographic methods, such as UPLC or GC and for valid mass identification I am using high-resolution mass spectrometry. The structural composition of Mo-cofactor containing hydroxylases is elucidated with heterologously produced enzymes enrichment enzymes via cryo electron-microscopy in collaboration with Petra Wendler, University of Potsdam. The structural information is  a prerequisite to link tailored enzymes to inorganic electrode materials (biohybrids) for biocatalytic processes with potential application for industrial processes.